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stain nuclei  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc stain nuclei
    Stain Nuclei, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stain+nuclei/DAPI/pm41496357-126-51-53
    Average 98 stars, based on 2136 article reviews
    stain nuclei - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Cannflavin B ameliorates behavioural and neuronal systems alterations in adolescent rats exposed to prenatal valproic acid.
    Article Snippet: Sections were first washed in 1X Tris-Buffered Saline (TBS, 60.5 mM Tris, 87.6 mM NaCl pH 7.6), blocked for 2 h (10 % goat serum, 1 % BSA, 0.2 % Triton-X, 1X TBS), and incubated with mouse anti-Iba1 (ionized calcium-binding adaptor molecule 1 (Iba1), 1:200; Abcam, AB283319) primary antibody for 60 h at 4 oC. .. Following incubation, sections were washed in 1X TBS, blocked (5 % goat serum, 0.5 % BSA, 0.01 % Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 h. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI (4’,6-diamidino-2-phenylindole) was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).

    Staining:

    Article Title: Cannflavin B ameliorates behavioural and neuronal systems alterations in adolescent rats exposed to prenatal valproic acid.
    Article Snippet: Sections were first washed in 1X Tris-Buffered Saline (TBS, 60.5 mM Tris, 87.6 mM NaCl pH 7.6), blocked for 2 h (10 % goat serum, 1 % BSA, 0.2 % Triton-X, 1X TBS), and incubated with mouse anti-Iba1 (ionized calcium-binding adaptor molecule 1 (Iba1), 1:200; Abcam, AB283319) primary antibody for 60 h at 4 oC. .. Following incubation, sections were washed in 1X TBS, blocked (5 % goat serum, 0.5 % BSA, 0.01 % Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 h. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI (4’,6-diamidino-2-phenylindole) was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).

    Article Title: Cannflavin B ameliorates social and anxiety deficits and neuronal systems dysfunction in adolescent rats exposed to prenatal valproic acid
    Article Snippet: Following incubation, sections were washed in 1X TBS, blocked (5% goat serum, 0.5% BSA, 0.01% Triton-X, 1X TBS), and incubated in an anti-mouse-Alexa 488 (1:200; Invitrogen) secondary antibody for 2 hours. .. Slices were then washed, and on the last wash 0.4 μg/mL of DAPI was added to stain nuclei (Cell Signaling, #4083). .. Brain slices were then mounted on slides using Prolong Gold (Thermo Fisher Scientific).



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